Human/Mouse RPS6 (S235/S236) Cell-Based ELISA Kit

RayBio® Cell-Based Human/Mouse RPS6 (S235/S236) Phosphorylation ELISA Kit. Suitable for adherent whole cell lines.

Human/Mouse RPS6 (S235/S236) Cell-Based ELISA Kit

RayBio® Cell-Based Human/Mouse RPS6 (S235/S236) Phosphorylation ELISA Kit. Suitable for adherent whole cell lines.

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	1-2 business days
Species	Human, Mouse
Specificity	The antibodies provided in this kit recognizes human/mouse/rat RPS6 phosphorylated at Serine-235 / Serine-236 and total RPS6 for comparison.
Compatible Sample Types	Adherent Cell Culture
Accession Number	P62753
Gene Id	6194
Gene Symbols	RPS6, OK/SW-CL.2
Protein Name / Synonyms	40S ribosomal protein S6 (Phosphoprotein NP33)
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	96-well Microplate
Design Principle	Cell-based
Method Of Detection	Colorimetric
Research Area	Post-Translational Modifications, Phosphorylation, PI3K-AKT Signaling, Translation
Shipping Type	Blue ice
Storage	-20°C

Product Features

Site and signal pathway-specific
In vitro detection of adherent cell culture
No sample lysis is needed
Compatible with a standard ELISA plate reader
Faster results than with ELISA
Adaptable for high-throughput screening and drug discovery

Application Notes

Kit Components

uncoated 96-well Microplate

Wash Buffer A

Wash Buffer B

Fixing Solution

Quenching Buffer

Blocking Buffer

Anti-phospho antibody

Anti-pan antibody

HRP-Conjugated Secondary Antibody

TMB One-Step Substrate

Stop Solution

Other Materials Required

Distilled or deionized water
100 ml and 1 liter graduated cylinders
Tubes to prepare sample dilutions
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
Benchtop rocker or shaker
Microplate reader capable of measuring absorbance at 450 nm

Protocol Outline

Seed 10,000-30,000 cells into each well and incubate overnight.
Apply various treatment, inhibitors or activators according to manufacture's instructions.
Add 100 µl of Fixing Solution into each well and incubate for 20 min at RT with shaking.
Add 200 µl of prepared 1X Quenching Buffer and incubate 20 min at RT.
Add 200 µl of Blocking Solution and incubate for 1 h at 37 °C.
Add 50 µl of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
Add 50 µl of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20^°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

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Get your lot-specific datasheet at Ray's Docs. The datasheet linked below is our current version and may not match your lot.

Datasheet (CBEL-RPS6.pdf)

SDS (393079754b49ce38bff5b3abca74c9c8657d7012.pdf)

Product Features

Site and signal pathway-specific
In vitro detection of adherent cell culture
No sample lysis is needed
Compatible with a standard ELISA plate reader
Faster results than with ELISA
Adaptable for high-throughput screening and drug discovery

Application Notes

Kit Components

uncoated 96-well Microplate

Wash Buffer A

Wash Buffer B

Fixing Solution

Quenching Buffer

Blocking Buffer

Anti-phospho antibody

Anti-pan antibody

HRP-Conjugated Secondary Antibody

TMB One-Step Substrate

Stop Solution

Other Materials Required

Distilled or deionized water
100 ml and 1 liter graduated cylinders
Tubes to prepare sample dilutions
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
Benchtop rocker or shaker
Microplate reader capable of measuring absorbance at 450 nm

Protocol Outline

Seed 10,000-30,000 cells into each well and incubate overnight.
Apply various treatment, inhibitors or activators according to manufacture's instructions.
Add 100 µl of Fixing Solution into each well and incubate for 20 min at RT with shaking.
Add 200 µl of prepared 1X Quenching Buffer and incubate 20 min at RT.
Add 200 µl of Blocking Solution and incubate for 1 h at 37 °C.
Add 50 µl of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
Add 50 µl of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20^°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Human/Mouse RPS6 (S235/S236) Cell-Based ELISA Kit

Human/Mouse RPS6 (S235/S236) Cell-Based ELISA Kit

Product Description

Specifications

Product Features

Application Notes

Storage/Stability

Have this product in hand?

Human/Mouse RPS6 (S235/S236) Cell-Based ELISA Kit

Human/Mouse RPS6 (S235/S236) Cell-Based ELISA Kit

Product Description

Specifications

Product Features

Application Notes

Storage/Stability

Have this product in hand?

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Human/Mouse RPS6 (S235/S236) Cell-Based ELISA Kit

Human/Mouse RPS6 (S235/S236) Cell-Based ELISA Kit

Product Description

Specifications

Product Features

Application Notes

Storage/Stability

Documents

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Human/Mouse RPS6 (S235/S236) Cell-Based ELISA Kit

Human/Mouse RPS6 (S235/S236) Cell-Based ELISA Kit

Product Description

Specifications

Related Products

Product Features

Application Notes

Storage/Stability

Documents

Have this product in hand?

Reviews

Ask A Question

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Phospho-RPS6 (S235/S236) ELISA Kit

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Phospho-RPS6 (S235/S236) ELISA Kit

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About Us

Contact Us

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