Human TGF beta Array C2

RayBio® C-Series Human TGF beta Array C2 Kit. Detects 25 proteins in human samples. Suitable for all liquid sample types but intended for use with cell and tissue lysates.

$345.00  
AAH-TGFB-2-2
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Antigen Information

Gene Symbols:
ACVR1 | BAMBI | BMP15 | BMP2 | BMP4 | BMP6 | BMP7 | BMP8A | BMPR1A | BMPR2   View more

Assay Format

Number of Targets Detected:
25
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Activin A
Activin R1A
(ALK-2)
BAMBI
(NMA)
beta IG-H3
BMP-2
BMP-4
BMP-6
BMP-7
BMP-8
BMP-9
BMP-15
(GDF-9B)
BMPR-IA
(ALK-3)
BMPR-II
Follistatin
GDF-11
(BMP-11)
GDF-15
Lefty-A
Noggin
  • Gene Symbol: NOG
  • Gene ID: 9241
  • Accession #: Q13253
SMAD3
SMAD4
TGF alpha
TGF beta 1
TGF beta 2
TGF-beta RII
TGF beta R3

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human TGF beta Array C2 Membranes
  • Blocking Buffer
  • Biotinylated Antibody Cocktail
  • 1,000X HRP-Streptavidin Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentrate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray w/ Lid
  • Protease Inhibitor Cocktail
  • Plastic Sheets
  • Array Map Template
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated anti-IgG
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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