Recombinant SARS-CoV-2 S1 Subunit Protein (RBD), mutant N331Q

Recombinant SARS-CoV-2 S1 subunit protein (RBD) mutant N331Q with C-terminal His-tag, derived from the transfected human HEK293 cells.

$312.90  
230-30157-100
+

Product Specifications

Size:
100 ug or 500 ug
Format:
Liquid
Formulation:
Supplied as a 0.2 um filtered solution in PBS (pH 7.4)
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration:
Lot specific (see the label on the vial), determined by BCA protein assay
Purity:
>95 %
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining

Source

Species:
SARS-CoV-2
Accession Number:
  • QHD43416
Expressed Region:
Arg319-Phe541. N-glycosylation site at Asn (N) 331 was mutated to Gln (Q).
Protein Name & Synonyms:
Spike protein, S Protein, S1 Subunit, Host Cell Receptor Binding Domain (RBD)

Preparation

Expression System:
Human embryonic kidney 293 (HEK293) cells
Tag:
His
Conjugation/Tag Notes:
C-terminal his-tag
Molecular Weight (kDa):
Recombinant protein product has a calculated molecular mass of 25 kDa. Due to the abundant glycosylation, it migrates as approximately 30 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below.
SDS-PAGE Image
SDS-PAGE
Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with Protein Deglycosylation Kit under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.
Lane 1: Protein standard ladder (kDa)
Lane 2: Untreated protein under reducing conditions
Lane 3: Treated protein with deglycosylation enzymes under native conditions
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.
Binding Function
Binding Function
Product binding ability was measured by ELISA. The immobilized recombinant human ACE2 protein (Catalog #230-30165, coated at 0.5 ug/ml, 100 ul/well) was incubated with the serial diluted SARS CoV-2 S1 RBD N331 mutant protein (Catalog # 230-30157). The bound mutant protein was detected by mouse anti-S1 RBD monoclonal antibody using ELISA. The calculated EC50 is 138.1 ng/ml.

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

Related Coronavirus Proteins

Spike Protein, S1 Subunit, Host Cell RBD
Spike Protein, S2 Subunit
Nucleocapsid Protein
S1 Subunit Protein (RBD) with C-terminal Fc tag
S1 Subunit Protein (RBD) with C-terminal His-tag
S1 Subunit Protein with C-terminal His-tag
S2 Subunit Protein with C-terminal His-tag
Nucleocapsid Protein with C-terminal His-tag

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.