Membrane Antibody Arrays: C-Series™
Simultaneously screen and compare expression levels of multiple cytokines, growth factors, proteases, soluble receptors, and other proteins from a wide variety of sample types with a membrane-based RayBiotech C-Series array.
Because C-Series arrays enable semi-quantitative measurement of multiple proteins at once, you can move your studies forward quickly and with the confidence of a single study instead of spending extra time, samples, and effort—and introducing additional variability—when you normalize and compare multiple experiments.
In addition, you can rely on the high quality and consistency of every C-Series array thanks to the stringent manufacturing and quality control processes we implement in our US-based manufacturing facilities.
What are C-Series arrays?
Each C-Series array consists of antibodies to cytokines, growth factors, proteases, soluble receptors, and other proteins immobilized onto a nitrocellulose membrane. The array is a semi-quantitative, sandwich-based assay that can report on between 10 and 274 different proteins and uses the same familiar workflow as a typical chemiluminescent Western blot.
What’s in each C-Series kit?
Each C-Series Antibody Array kit includes the key reagents for your assay:
- RayBio® C-Series Antibody Array Membranes
- Biotinylated Detection Antibody Cocktail
- Blocking Buffer
- Wash Buffers 1 and 2
- Cell & Tissue Lysis Buffer
- Detection Buffers C and D
- Plastic Incubation Tray
- Protease Inhibitor Cocktail (in select kits)
- User manual
How It Works
C-Series membrane arrays use a sandwich immunoassay design and the same workflows as a typical Western blot.
- Each array includes capture antibodies immobilized onto a nitrocellulose membrane.
- Incubate your sample with the array to enable binding of target proteins to the corresponding antibody.
- Detect bound protein by adding a cocktail of biotinylated antibodies.
- Visualize with labeled streptavidin using chemiluminescence.
You can use the same chemiluminescence detection systems developed for western blot visualization to measure the chemiluminescence of your C-Series membrane arrays. The entire procedure can be completed in 1 day and is simple enough that a novice researcher can successfully collect data with very few pitfalls and little or no optimization.
How to Handle C-Series Membrane Antibody Arrays
Need a deeper understanding of your array data?
Whether you’re looking for statistically significant differences between groups or wish to conduct a detailed protein-protein interaction (PPI) analysis, our in-house biostatistics and bioinformatics experts can build a tailored computational solution for your antibody and protein array data. Learn more>
- High Sensitivity: Detect as little as 1 pg/ml, depending on the protein.
- High Reproducibility: Inter-array coefficient of variation (CV) of spot signal intensities is as low as 5% when run under optimal conditions.
- High Specificity: Each antibody spot is tested using a recombinant protein to ensure that only a single spot is visible after hybridization.
- High Quality: After screening multiple antibody pairs for each protein target, only the pairs with the strongest affinity and sensitivity are selected for the array.
- Wide Dynamic Range: Many protein expression levels can be detected across as much as 4 orders of magnitude (104).
- Smart Array Design
- Positive control spots facilitate membrane orientation and normalization of results.
- Negative control and blank spots enable baseline and background measurement.
- Duplicate spots provide technical replicates on the same array, preserving limited samples while still enabling robust data generation.
- Proven Technology: There are hundreds of publications using C-Series antibody arrays.
- Small Membrane Size: Average membrane dimensions only 2.5 X 3.0 cm.
We’ve seen our membrane antibody arrays used to address a large range of research questions. Here are a few of our favorites:
- High-throughput profiling of cytokine expression.
- Identifying potential molecular targets for drug development.
- Identifying the molecular mechanisms of drug action.
- Identifying crucial factors involved in disease processes.
- Discovering biomarkers for disease management.
- Discovering expression patterns for molecular classification of diseases.
Don’t see what you need? Create a custom array
Tell us your analytes and objectives and we can design an array to meet your needs. Whether you need help selecting the best format or already know exactly what you need, our experienced scientists are ready to get your project started.