Product Description

Specifications

Size	48 tests, 96 tests, 192 tests, 480 tests
Estimated Lead Time	1-2 business days
Species	Mouse
Compatible Sample Types	Cell Culture Supernatants, Serum
Quantitative/Semi-Quantitative	Quantitative
Number of Targets Detected	13
Solid Support	Fluorescent Microbeads
Design Principle	Sandwich-based
Method Of Detection	Flow Cytometry
Research Area	Inflammation
Shipping Type	Blue ice
Storage	Mixed storage. See datasheet.

Product Features

Less sample, more data: up to 25 proteins can be quantified from only 25 µl or less of your precious sample
4 hour processing time
5-10x more cost-effective than ELISA
Compatible with most flow cytometers
No specialized or dedicated instrument(s) needed
High specificity and system reproducibility
Well-suited for high throughput assays

Target Names

Scroll over each target protein for more information
GCSF Gene Symbol: Csf3 Csfg Gene ID: 12985 Accession #: P09920 ELISA Kit: RayBio Mouse G-CSF ELISA	IFN-gamma Gene Symbol: Ifng Gene ID: 15978 Accession #: P01580 ELISA Kit: RayBio Mouse IFN-gamma ELISA	IL-10 Gene Symbol: Il10 Il-10 Gene ID: 16153 Accession #: P18893 ELISA Kit: RayBio Mouse IL-10 ELISA	IL-12 p70 Gene Symbol: Il12a Gene ID: 16159 Accession #: P43431 ELISA Kit: RayBio	IL-17A Gene Symbol: Il17a Ctla8 Il17 Gene ID: 16171 Accession #: Q62386 ELISA Kit: RayBio Mouse IL-17A ELISA
IL-1 beta (IL-1 F2) Gene Symbol: Il1b Gene ID: 16176 Accession #: P10749 ELISA Kit: RayBio Mouse IL-1 beta ELISA	IL-2 Gene Symbol: Il2 Il-2 Gene ID: 16183 Accession #: P04351 ELISA Kit: RayBio Mouse IL-2 ELISA	IL-23 p19 Gene Symbol: Il23a Gene ID: 83430 Accession #: Q9EQ14 ELISA Kit: RayBio Mouse IL-23 ELISA	IL-4 Gene Symbol: Il4 Il-4 Gene ID: 16189 Accession #: P07750 ELISA Kit: RayBio Mouse IL-4 ELISA	IL-6 Gene Symbol: Il6 Il-6 Gene ID: 16193 Accession #: P08505 ELISA Kit: RayBio Mouse IL-6 ELISA
KC (CXCL1) Gene Symbol: Cxcl1 Gro Gro1 Mgsa Scyb1 Gene ID: Cxcl1 Gro Gro1 Mgsa Scyb1 Accession #: P12850 ELISA Kit: RayBio	MCP-1 (CCL2) Gene Symbol: Ccl2 Je Mcp1 Scya2 Gene ID: 20296 Accession #: P10148 ELISA Kit: RayBio Mouse MCP-1 ELISA	TNF alpha Gene Symbol: Tnf Tnfa Tnfsf2 Gene ID: 21926 Accession #: P06804 ELISA Kit: RayBio Mouse TNF-alpha ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

RayPlex™ Multiplex Bead Cocktail
1X Assay Diluent
20X Wash Buffer
Lyophilized Protein Standard Mix
Biotinylated Detection Antibody Cocktail
10X PE Conjugate
Flow Cytometer Setup Bead Cocktail
V-bottom 96-well Microplate
Filter 96-well Microplate
RayBio® Serum Diluent

Other Materials Required

Orbital 96-well plate shaker (with ability to reach 1000 rpm)
Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
Aluminum foil or 96-well Plate Aluminum Sealers
Distilled water
1.5 ml polypropylene microcentrifuge tubes or similar
Microcentrifuge
If using the Filter 96-Well Microplate (Item 11): Vacuum manifold compatible with a 96-well microplate
Optional: 96-well round bottom plate (to prepare samples)
Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!

Protocol Outline

Prepare Reagents, Standards, and Samples
Add RayPlex™ Multiplex Bead Cocktail to each well
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluorophore
Analyze with a compatible flow cytometer
Perform data analysis

Storage/Stability

Upon receipt, all components should be stored at 4°C. The Lyophilized Protein Standard Mix (Item 4) should be stored at 4°C or below. Use reagents immediately after preparation. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.

Product Features

Less sample, more data: up to 25 proteins can be quantified from only 25 µl or less of your precious sample
4 hour processing time
5-10x more cost-effective than ELISA
Compatible with most flow cytometers
No specialized or dedicated instrument(s) needed
High specificity and system reproducibility
Well-suited for high throughput assays

Target Names

Scroll over each target protein for more information
GCSF Gene Symbol: Csf3 Csfg Gene ID: 12985 Accession #: P09920 ELISA Kit: RayBio Mouse G-CSF ELISA	IFN-gamma Gene Symbol: Ifng Gene ID: 15978 Accession #: P01580 ELISA Kit: RayBio Mouse IFN-gamma ELISA	IL-10 Gene Symbol: Il10 Il-10 Gene ID: 16153 Accession #: P18893 ELISA Kit: RayBio Mouse IL-10 ELISA	IL-12 p70 Gene Symbol: Il12a Gene ID: 16159 Accession #: P43431 ELISA Kit: RayBio	IL-17A Gene Symbol: Il17a Ctla8 Il17 Gene ID: 16171 Accession #: Q62386 ELISA Kit: RayBio Mouse IL-17A ELISA
IL-1 beta (IL-1 F2) Gene Symbol: Il1b Gene ID: 16176 Accession #: P10749 ELISA Kit: RayBio Mouse IL-1 beta ELISA	IL-2 Gene Symbol: Il2 Il-2 Gene ID: 16183 Accession #: P04351 ELISA Kit: RayBio Mouse IL-2 ELISA	IL-23 p19 Gene Symbol: Il23a Gene ID: 83430 Accession #: Q9EQ14 ELISA Kit: RayBio Mouse IL-23 ELISA	IL-4 Gene Symbol: Il4 Il-4 Gene ID: 16189 Accession #: P07750 ELISA Kit: RayBio Mouse IL-4 ELISA	IL-6 Gene Symbol: Il6 Il-6 Gene ID: 16193 Accession #: P08505 ELISA Kit: RayBio Mouse IL-6 ELISA
KC (CXCL1) Gene Symbol: Cxcl1 Gro Gro1 Mgsa Scyb1 Gene ID: Cxcl1 Gro Gro1 Mgsa Scyb1 Accession #: P12850 ELISA Kit: RayBio	MCP-1 (CCL2) Gene Symbol: Ccl2 Je Mcp1 Scya2 Gene ID: 20296 Accession #: P10148 ELISA Kit: RayBio Mouse MCP-1 ELISA	TNF alpha Gene Symbol: Tnf Tnfa Tnfsf2 Gene ID: 21926 Accession #: P06804 ELISA Kit: RayBio Mouse TNF-alpha ELISA

Application Notes

Suggested Application

Kit Components

RayPlex™ Multiplex Bead Cocktail
1X Assay Diluent
20X Wash Buffer
Lyophilized Protein Standard Mix
Biotinylated Detection Antibody Cocktail
10X PE Conjugate
Flow Cytometer Setup Bead Cocktail
V-bottom 96-well Microplate
Filter 96-well Microplate
RayBio® Serum Diluent

Other Materials Required

Orbital 96-well plate shaker (with ability to reach 1000 rpm)
Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
Aluminum foil or 96-well Plate Aluminum Sealers
Distilled water
1.5 ml polypropylene microcentrifuge tubes or similar
Microcentrifuge
If using the Filter 96-Well Microplate (Item 11): Vacuum manifold compatible with a 96-well microplate
Optional: 96-well round bottom plate (to prepare samples)
Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!

Protocol Outline

Prepare Reagents, Standards, and Samples
Add RayPlex™ Multiplex Bead Cocktail to each well
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluorophore
Analyze with a compatible flow cytometer
Perform data analysis

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