The nucleocapsid protein (N-protein) and spike protein (S-protein) are encoded by all coronaviruses, including the coronavirus (SARS-CoV-2, COVID-19) that was first detected in Wuhan City, China, in December 2019. Both recombinant forms of these proteins are now available from RayBiotech to advance infectious disease research (Figures 1 & 2).
The nucleocapsid protein (N-protein) is a structural protein that binds to the coronavirus RNA genome, thus creating a shell (or capsid) around the enclosed nucleic acid. The N-protein also 1) interacts with the viral membrane protein during viral assembly, 2) assists in RNA synthesis and folding, 3) plays a role in virus budding, and 4) affects host cell responses, including cell cycle and translation.
The spike protein (S-protein) performs two primary tasks that aid in host infection: 1) mediates the attachment between the virus and host cell surface receptors, and 2) facilitates viral entry into the host cell by assisting in the fusion of the viral and host cell membranes.
ACE2 is an endogenous membrane protein that enables COVID-19 infection. During infection, the extracellular peptidase domain of ACE2 binds to the receptor binding domain of spike protein, which is a surface protein on SARS-CoV-2.
|Protein||Catalog #||Protein Domain||Purification||Expression Host||Expression Region||Tag||MW (kDa)|
|N protein, Nucleocapsid||230-30164||Full length||Purified||HEK293 Cell||Met1 - Ala419||C-terminal His-tag||⁓50 kDa|
|230-01104||Purified||E.coli||Met1 - Ala419||N-terminal His-tag||⁓50 kDa|
|230-20409||Unpurified||HEK293 Cell||Met1 - Ala419||C-terminal His-tag||⁓50 kDa|
|S Protein, Spike||230-01102||S1 subunit, RBD||Purified||E.coli||Arg319 - Phe541||N-terminal His-tag||⁓25 kDa|
|230-30162||Purified||HEK293 Cell||Arg319 - Phe541||C-terminal His-tag||⁓25 kDa|
|230-20406||Unpurified||HEK293 Cell||Arg319 - Phe541||C-terminal His-tag||⁓25 kDa|
|230-20405||Unpurified||HEK293 Cell||Arg319 - Phe541||C-terminal Fc-tag||⁓50 kDa|
|230-01101||S1 subunit, full-length||Purified||E.coli||Val16 - Gln690||N-terminal His-tag||⁓75 kDa|
|230-20407||Unpurified||HEK293 Cell||Val16 - Gln690||C-terminal His-tag||⁓75 kDa|
|230-01103||S2 subunit, full-length||Purified||E.coli||Met697 - Pro1213||N-terminal His-tag||⁓58 kDa|
|230-20408||Unpurified||HEK293 Cell||Met697 - Pro1213||C-terminal His-tag||⁓60 kDa|
|Human ACE2||230-30165||Full-length||Purified||HEK293 Cell||Gln18-Ser740||C-terminal His-tag||⁓90 kDa|
For help identifying the right protein for your research, please contact our technical support team at [email protected] or (888) 494-8555.