Human Phosphorylation Pathway Profiling Array C55

RayBio® C-Series Human Phosphorylation Pathway Profiling Array C55 Kit. Detects phosphorylated human proteins in 5 signaling pathways: MAPK, AKT, JAK/STAT, NFκB, and TGFβ. Suitable for all liquid sample types but intended for use with cell and tissue lysates.


Assay Format

  • Akt Signaling
  • JAK/STAT Signaling
  • MAPK Signaling
  • NFkB Signaling
  • TGF-beta Signaling
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Semi-Quantitative
Solid Support:

Product Specifications

2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
AKT1 | AKT1S1 | AMPK1 | AND | ATF2 | ATM | BAD | CDKN1B | CREB1 | EGFR   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
4E-BP1 (P-Thr36)
AKT (P-Ser473)
AMPKa (P-Thr172)
  • Gene Symbol: AMPK1
  • Gene ID: 5562
  • Accession #: P54645
  • ELISA Kit: RayBio
ATF2 (P-Thr69/71)
ATM (P-Ser1981)
BAD (P-Ser112)
C-Fos (P-Thr232)
c-Jun (P-Ser73)
  • Gene Symbol: JUN
  • Gene ID: 3725;
  • Accession #: P05412
  • ELISA Kit: RayBio
CREB (P-Ser133)
EGFR (P-Ser1070)
eIF-2a (P-Ser51)
  • Gene Symbol: EIF2S1 EIF2A
  • Gene ID: 1965;
  • Accession #: P05198
  • ELISA Kit: RayBio
ERK1/2 (P-Thr202/P-Tyr204/P-Tyr185/P-Tyr187)
GSK-3 alpha (P-Ser21)
GSK-3 beta (P-Ser9)
HDAC2 (P-Ser394)
HDAC4 (P-Ser632)
HSP27 (P-Ser82)
IKBa (P-Ser32)
JAK1 (P-Tyr1022)
JAK2 (P-Tyr1007/1008)
JNK (P-Thr183)
MEK (P-Ser217/221)
MKK3 (P-Ser189)
MKK6 (P-Ser207)
MSK1 (P-Ser376)
MSK2 (P-Ser360)
mTOR (P-Ser2448)
NF-kB (P-Ser536)
p27 (P-Thr198)
p38 (P-Thr180/Tyr182)
P53 (P-Ser15)
P70S6K (P-Thr421/Ser424)
PDK1 (P-Ser241)
PRAS40 (P-Thr246)
PTEN (P-Ser380)
Raf-1 (P-Ser301)
RPS6 (P-Ser235/236)
RSK1 (P-Ser380)
RSK2 (P-Ser386)
SHP-1 (P-Ser591)
SHP2 (P-Tyr542)
SMAD1 (P-Ser463/465)
SMAD2 (P-Ser245/250/255)
SMAD4 (P-Thr277)
SMAD5 (P-Ser463/465)
Src (P-Tyr419)
STAT1 (P-Ser727)
STAT2 (P-Tyr689)
STAT3 (P-Tyr705)
STAT5 (P-Tyr694)
STAT6 (P-Tyr641)
TAK1 (P-Ser412)
TBK1 (P-Ser172)
TYK2 (P-Tyr1054)
ZAP70 (P-Tyr292)

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Phosphorylation Pathway Profiling Array C55 Membranes
  • Blocking Buffer
  • Detection Antibody Cocktail
  • 500X HRP-Anti-Rabbit IgG Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentrate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray w/ Lid
  • Protease Inhibitor Cocktail
  • 100x Phosphatase Inhibitor Cocktail I
  • Phosphatase Inhibitor Cocktail II
  • Plastic Sheets
  • Array Map Template
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated anti-IgG
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis


For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
It was easy to test multiple phosphorylation targets with limited sample quantity and workflow was really easy and uncomplicated. I used primary human endothelium cells with 100 ug of protein for each array and signals were good with 10-sec exposure.

University of Wisconsin-Madison
This kit works pretty well for my samples. I used 700 ug of my samples (cell lysates) and performed exactly according to the instructional protocol and I got a very strong signal. The only reason that I didn't give it 5 stars was we had to purchase their software to analyze the results. As we already spent nearly $1000, we should be able to use the software for free, at least one time free.

Kalya Pharma
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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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