This multiplex phospho-array detects 55 phosphorylated human proteins from 5 signaling pathways: MAPK, AKT, JAK/STAT, NFκB, and TGFβ. Suitable for all liquid sample types, but intended for use with cell and tissue lysates.
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 55 |
| Recommended Applications | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Research Area | Post-Translational Modifications, Phosphorylation, JAK/STAT Signaling, MAPK Signaling, PI3K-AKT Signaling, TGF-beta Signaling, NFkB Signaling, Akt Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
| Scroll over each target protein for more information | ||||
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4E-BP1 (P-Thr36)
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AKT (P-Ser473)
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ATF2 (P-Thr69/71)
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ATM (P-Ser1981)
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BAD (P-Ser112)
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C-Fos (P-Thr232)
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CREB (P-Ser133)
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EGFR (P-Ser1070)
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ERK1/2 (P-Thr202/P-Tyr204/P-Tyr185/P-Tyr187)
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GSK-3 alpha (P-Ser21)
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GSK-3 beta (P-Ser9)
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HDAC2 (P-Ser394)
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HDAC4 (P-Ser632)
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HSP27 (P-Ser82)
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IKBa (P-Ser32)
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JAK1 (P-Tyr1022)
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JAK2 (P-Tyr1007/1008)
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JNK (P-Thr183)
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MEK (P-Ser217/221)
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MKK3 (P-Ser189)
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MKK6 (P-Ser207)
(MEK6)
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MSK1 (P-Ser376)
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MSK2 (P-Ser360)
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mTOR (P-Ser2448)
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NF-kB (P-Ser536)
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p27 (P-Thr198)
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p38 (P-Thr180/Tyr182)
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P53 (P-Ser15)
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P70S6K (P-Thr421/Ser424)
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PDK1 (P-Ser241)
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PRAS40 (P-Thr246)
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PTEN (P-Ser380)
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Raf-1 (P-Ser301)
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RPS6 (P-Ser235/236)
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RSK1 (P-Ser380)
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RSK2 (P-Ser386)
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SHP-1 (P-Ser591)
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SHP2 (P-Tyr542)
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SMAD1 (P-Ser463/465)
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SMAD2 (P-Ser245/250/255)
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SMAD4 (P-Thr277)
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SMAD5 (P-Ser463/465)
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Src (P-Tyr419)
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STAT1 (P-Ser727)
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STAT2 (P-Tyr689)
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STAT3 (P-Tyr705)
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STAT5 (P-Tyr694)
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STAT6 (P-Tyr641)
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TAK1 (P-Ser412)
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TBK1 (P-Ser172)
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TYK2 (P-Tyr1054)
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ZAP70 (P-Tyr292)
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This multiplex phospho-array detects 55 phosphorylated human proteins from 5 signaling pathways: MAPK, AKT, JAK/STAT, NFκB, and TGFβ. Suitable for all liquid sample types, but intended for use with cell and tissue lysates.
RayBiotech’s Human Phosphorylation Pathway Profiling Array identified oncogenic pathway alterations in glioblastoma cells overexpressing sst5TMD4. Figure adapted from Fuentes-Fayos et al., Int. J. Mol. Sci., 2022.
RayBiotech’s Human Phosphorylation Pathway Profiling Array identified oncogenic pathway alterations in glioblastoma cells overexpressing sst5TMD4. Figure adapted from Fuentes-Fayos et al., Int. J. Mol. Sci., 2022.
It was easy to test multiple phosphorylation targets with limited sample quantity and workflow was really easy and straightforward. I used primary human PBMCs with 100 ug of protein for each array and signals were good with 20-sec exposure.
Review by Jason, from Frontage laboratories, on Jun 21, 2025
This kit worked really well. The kit came with everything you would need to run the experiment. I used the minimum recommended amount of protein and it worked very well. I did have a couple issues, but that wouldn't stop me from ordering it again. First, I tried marking the membranes at the their recommendation, but the ink I used bled onto a few of the protein dots so just be aware that it may happen. Also, the analysis software was a bit of pain. For each pathway, there was a specific excel file (so 5 total) and some were updated and some were not. It would have been easier to have everything in one file that also didn't have several tabs for the analysis, or at least a much more organized file with clear description of each step. I ended up doing the analysis on my own after struggling for a bit. But overall, very happy with my purchase.
Review by Charles Kerr, on Apr 25, 2023
easy to use, with clear results at the end. I was using human fibroblasts to investigate the affect of Crohn's disease serum on downstream pathways, and worked well with less than recommended protein concentration due to low concentration from the samples
Review by Sophie, on Apr 5, 2023
It was easy to test multiple phosphorylation targets with limited sample quantity and workflow was really easy and uncomplicated. I used primary human endothelium cells with 100 ug of protein for each array and signals were good with 10-sec exposure.
Review by Jay Mishra, from University of Wisconsin-Madison, on Jul 4, 2021
This kit works pretty well for my samples. I used 700 ug of my samples (cell lysates) and performed exactly according to the instructional protocol and I got a very strong signal. The only reason that I didn't give it 5 stars was we had to purchase their software to analyze the results. As we already spent nearly $1000, we should be able to use the software for free, at least one time free.
Review by Jianhui Ma, from Kalya Pharma, on Jul 14, 2019
