Product Description

Specifications

Size	48 tests, 96 tests, 192 tests, 480 tests
Estimated Lead Time	1-2 business days
Species	Human
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Quantitative/Semi-Quantitative	Quantitative
Number of Targets Detected	14
Solid Support	Fluorescent Microbeads
Design Principle	Sandwich-based
Method Of Detection	Flow Cytometry
Shipping Type	Blue ice
Storage	Mixed storage. See datasheet.

Product Features

Less sample, more data: up to 25 proteins can be quantified from only 25 µl or less of your precious sample
4 hour processing time
5-10x more cost-effective than ELISA
Compatible with most flow cytometers
No specialized or dedicated instrument(s) needed
High specificity and system reproducibility
Well-suited for high throughput assays

Target Names

Scroll over each target protein for more information
GM-CSF Gene Symbol: CSF2 GMCSF Gene ID: 1437 Accession #: P04141 ELISA Kit: RayBio Human GM-CSF ELISA	IFN-gamma Gene Symbol: IFNG Gene ID: 3458 Accession #: P01579 ELISA Kit: RayBio Human IFN-gamma ELISA	IL-1 beta (IL-1 F2) Gene Symbol: IL1B IL1F2 Gene ID: 3553 Accession #: P01584 ELISA Kit: RayBio Human IL-1 beta ELISA	IL-10 Gene Symbol: IL10 Gene ID: 3586 Accession #: P22301 ELISA Kit: RayBio Human IL-10 ELISA	IL-12 p70 Gene Symbol: IL12A NKSF1 Gene ID: 3592 Accession #: P29459 ELISA Kit: RayBio Human IL-12 p70 ELISA
IL-15 Gene Symbol: IL15 Gene ID: 3600 Accession #: P40933 ELISA Kit: RayBio Human IL-15 ELISA	IL-18 Gene Symbol: IL18 IGIF IL1F4 Gene ID: 3606 Accession #: Q14116 ELISA Kit: RayBio Human IL-18 ELISA	IL-2 Gene Symbol: IL2 Gene ID: 3558 Accession #: P60568 ELISA Kit: RayBio Human IL-2 ELISA	IL-6 Gene Symbol: IL6 IFNB2 Gene ID: 3569 Accession #: P05231 ELISA Kit: RayBio Human IL-6 ELISA	IL-8 (CXCL8) Gene Symbol: CXCL8 IL8 Gene ID: 3576 Accession #: P10145 ELISA Kit: RayBio Human IL-8 ELISA
MIP-1 alpha (CCL3) Gene Symbol: CCL3 G0S19-1 MIP1A SCYA3 Gene ID: 6348 Accession #: P10147 ELISA Kit: RayBio Human MIP-1 alpha ELISA	MIP-1 beta (CCL4) Gene Symbol: CCL4 LAG1 MIP1B SCYA4 Gene ID: 388372 6351 Accession #: P13236 ELISA Kit: RayBio Human MIP-1 beta ELISA	RANTES (CCL5) Gene Symbol: CCL5 D17S136E SCYA5 Gene ID: 6352 Accession #: P13501 ELISA Kit: RayBio Human RANTES ELISA	TNF alpha Gene Symbol: TNF TNFA TNFSF2 Gene ID: 7124 Accession #: P01375 ELISA Kit: RayBio Human TNF-alpha ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

RayPlex™ Multiplex Bead Cocktail
1X Assay Diluent
20X Wash Buffer
RayBio^® Serum/Plasma Diluent
Lyophilized Protein Standard Mix
1X Biotinylated Detection Antibody Cocktail
10X PE Conjugate
Flow Cytometer Setup Bead Cocktail
V-bottom 96-well Microplate
Filter 96-well Microplate

Other Materials Required

Orbital 96-well plate shaker (with ability to reach 1000 rpm)
Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
Aluminum foil or 96-well Plate Aluminum Sealers
Distilled water
1.5 ml polypropylene microcentrifuge tubes or similar
Microcentrifuge
Vacuum manifold compatible with a 96-well microplate if using the Filter 96-Well Microplate
Optional: 96-well round bottom plate (to prepare samples)
Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!

Protocol Outline

Prepare Reagents, Standards, and Samples
Add RayPlex™ Multiplex Bead Cocktail to each well
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluorophore
Analyze with a compatible flow cytometer
Perform data analysis

Storage/Stability

Upon receipt, the Lyophilized Protein Standard Mix should be stored at -20°C or below. Other components should be stored at 4°C. Use reagents immediately after preparation. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.

Product Features

Less sample, more data: up to 25 proteins can be quantified from only 25 µl or less of your precious sample
4 hour processing time
5-10x more cost-effective than ELISA
Compatible with most flow cytometers
No specialized or dedicated instrument(s) needed
High specificity and system reproducibility
Well-suited for high throughput assays

Target Names

Scroll over each target protein for more information
GM-CSF Gene Symbol: CSF2 GMCSF Gene ID: 1437 Accession #: P04141 ELISA Kit: RayBio Human GM-CSF ELISA	IFN-gamma Gene Symbol: IFNG Gene ID: 3458 Accession #: P01579 ELISA Kit: RayBio Human IFN-gamma ELISA	IL-1 beta (IL-1 F2) Gene Symbol: IL1B IL1F2 Gene ID: 3553 Accession #: P01584 ELISA Kit: RayBio Human IL-1 beta ELISA	IL-10 Gene Symbol: IL10 Gene ID: 3586 Accession #: P22301 ELISA Kit: RayBio Human IL-10 ELISA	IL-12 p70 Gene Symbol: IL12A NKSF1 Gene ID: 3592 Accession #: P29459 ELISA Kit: RayBio Human IL-12 p70 ELISA
IL-15 Gene Symbol: IL15 Gene ID: 3600 Accession #: P40933 ELISA Kit: RayBio Human IL-15 ELISA	IL-18 Gene Symbol: IL18 IGIF IL1F4 Gene ID: 3606 Accession #: Q14116 ELISA Kit: RayBio Human IL-18 ELISA	IL-2 Gene Symbol: IL2 Gene ID: 3558 Accession #: P60568 ELISA Kit: RayBio Human IL-2 ELISA	IL-6 Gene Symbol: IL6 IFNB2 Gene ID: 3569 Accession #: P05231 ELISA Kit: RayBio Human IL-6 ELISA	IL-8 (CXCL8) Gene Symbol: CXCL8 IL8 Gene ID: 3576 Accession #: P10145 ELISA Kit: RayBio Human IL-8 ELISA
MIP-1 alpha (CCL3) Gene Symbol: CCL3 G0S19-1 MIP1A SCYA3 Gene ID: 6348 Accession #: P10147 ELISA Kit: RayBio Human MIP-1 alpha ELISA	MIP-1 beta (CCL4) Gene Symbol: CCL4 LAG1 MIP1B SCYA4 Gene ID: 388372 6351 Accession #: P13236 ELISA Kit: RayBio Human MIP-1 beta ELISA	RANTES (CCL5) Gene Symbol: CCL5 D17S136E SCYA5 Gene ID: 6352 Accession #: P13501 ELISA Kit: RayBio Human RANTES ELISA	TNF alpha Gene Symbol: TNF TNFA TNFSF2 Gene ID: 7124 Accession #: P01375 ELISA Kit: RayBio Human TNF-alpha ELISA

Application Notes

Suggested Application

Kit Components

RayPlex™ Multiplex Bead Cocktail
1X Assay Diluent
20X Wash Buffer
RayBio^® Serum/Plasma Diluent
Lyophilized Protein Standard Mix
1X Biotinylated Detection Antibody Cocktail
10X PE Conjugate
Flow Cytometer Setup Bead Cocktail
V-bottom 96-well Microplate
Filter 96-well Microplate

Other Materials Required

Orbital 96-well plate shaker (with ability to reach 1000 rpm)
Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
Aluminum foil or 96-well Plate Aluminum Sealers
Distilled water
1.5 ml polypropylene microcentrifuge tubes or similar
Microcentrifuge
Vacuum manifold compatible with a 96-well microplate if using the Filter 96-Well Microplate
Optional: 96-well round bottom plate (to prepare samples)
Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!

Protocol Outline

Prepare Reagents, Standards, and Samples
Add RayPlex™ Multiplex Bead Cocktail to each well
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluorophore
Analyze with a compatible flow cytometer
Perform data analysis

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RayPlex® Human Anti-Viral Bead Array (14-Plex)

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Documents

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Reviews

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