Product Description

Specifications

Size	48 tests, 96 tests, 192 tests, 480 tests
Estimated Lead Time	1-2 business days
Species	Human
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Quantitative/Semi-Quantitative	Quantitative
Number of Targets Detected	13
Solid Support	Fluorescent Microbeads
Design Principle	Sandwich-based
Method Of Detection	Flow Cytometry
Research Area	Stem Cell Biology
Shipping Type	Blue ice
Storage	Mixed storage. See datasheet.

Product Features

Less sample, more data: up to 25 proteins can be quantified from only 25 µl or less of your precious sample
4 hour processing time
5-10x more cost-effective than ELISA
Compatible with most flow cytometers
No specialized or dedicated instrument(s) needed
High specificity and system reproducibility
Well-suited for high throughput assays

Target Names

Scroll over each target protein for more information
Eotaxin-1 (CCL11) Gene Symbol: CCL11 SCYA11 Gene ID: 6356 Accession #: P51671 ELISA Kit: RayBio Human Eotaxin ELISA	GRO alpha (CXCL1) Gene Symbol: CXCL1 GRO GRO1 GROA MGSA SCYB1 Gene ID: 2919 Accession #: P09341 ELISA Kit: RayBio Human GRO-alpha ELISA	ICAM-1 (CD54) Gene Symbol: ICAM1 Gene ID: 3383 Accession #: P05362 ELISA Kit: RayBio Human sICAM-1 ELISA	IL-6 Gene Symbol: IL6 IFNB2 Gene ID: 3569 Accession #: P05231 ELISA Kit: RayBio Human IL-6 ELISA	IL-8 (CXCL8) Gene Symbol: CXCL8 IL8 Gene ID: 3576 Accession #: P10145 ELISA Kit: RayBio Human IL-8 ELISA
IL-10 Gene Symbol: IL10 Gene ID: 3586 Accession #: P22301 ELISA Kit: RayBio Human IL-10 ELISA	IP-10 (CXCL10) Gene Symbol: CXCL10 INP10 SCYB10 Gene ID: 3627 Accession #: P02778 ELISA Kit: RayBio Human IP-10 ELISA	MCP-1 (CCL2) Gene Symbol: CCL2 MCP1 SCYA2 Gene ID: 6347 Accession #: P13500 ELISA Kit: RayBio Human MCP-1 ELISA	MIP-1 alpha (CCL3) Gene Symbol: CCL3 G0S19-1 MIP1A SCYA3 Gene ID: 6348 Accession #: P10147 ELISA Kit: RayBio Human MIP-1 alpha ELISA	MIP-1 beta (CCL4) Gene Symbol: CCL4 LAG1 MIP1B SCYA4 Gene ID: 388372 6351 Accession #: P13236 ELISA Kit: RayBio Human MIP-1 beta ELISA
RANTES (CCL5) Gene Symbol: CCL5 D17S136E SCYA5 Gene ID: 6352 Accession #: P13501 ELISA Kit: RayBio Human RANTES ELISA	TGF beta 1 Gene Symbol: TGFB1 TGFB Gene ID: 7040 Accession #: P01137 ELISA Kit: RayBio Human TGF-beta 1 ELISA	VEGF-A Gene Symbol: VEGFA VEGF Gene ID: 7422 Accession #: P15692 ELISA Kit: RayBio Human VEGF-A ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

RayPlex Multiplex Bead Cocktail
1X Assay Diluent
20X Wash Buffer
RayBio^® Serum/Plasma Diluent
Lyophilized Protein Standard Mix
1X Biotinylated Detection Antibody Cocktail
10X PE Conjugate
Flow Cytometer Setup Bead Cocktail
V-bottom 96-well Microplate
Filter 96-well Microplate

Other Materials Required

Orbital 96-well plate shaker (with ability to reach 1000 rpm)
Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
Aluminum foil or 96-well Plate Aluminum Sealers
Distilled water
1.5 ml polypropylene microcentrifuge tubes or similar
Microcentrifuge
If using the Filter 96-Well Microplate (Item 11): Vacuum manifold compatible with a 96-well microplate
Optional: 96-well round bottom plate (to prepare samples)
Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex bead arrays. Get a quote!

Protocol Outline

Prepare Reagents, Standards, and Samples
Add RayPlex Multiplex Bead Cocktail to each well
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluorophore
Analyze with a compatible flow cytometer
Perform data analysis

Storage/Stability

Upon receipt, all components should be stored at 4°C. The Lyophilized Protein Standard Mix (Item 4) should be stored at 4°C or below. Use reagents immediately after preparation. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.

Product Features

Less sample, more data: up to 25 proteins can be quantified from only 25 µl or less of your precious sample
4 hour processing time
5-10x more cost-effective than ELISA
Compatible with most flow cytometers
No specialized or dedicated instrument(s) needed
High specificity and system reproducibility
Well-suited for high throughput assays

Target Names

Scroll over each target protein for more information
Eotaxin-1 (CCL11) Gene Symbol: CCL11 SCYA11 Gene ID: 6356 Accession #: P51671 ELISA Kit: RayBio Human Eotaxin ELISA	GRO alpha (CXCL1) Gene Symbol: CXCL1 GRO GRO1 GROA MGSA SCYB1 Gene ID: 2919 Accession #: P09341 ELISA Kit: RayBio Human GRO-alpha ELISA	ICAM-1 (CD54) Gene Symbol: ICAM1 Gene ID: 3383 Accession #: P05362 ELISA Kit: RayBio Human sICAM-1 ELISA	IL-6 Gene Symbol: IL6 IFNB2 Gene ID: 3569 Accession #: P05231 ELISA Kit: RayBio Human IL-6 ELISA	IL-8 (CXCL8) Gene Symbol: CXCL8 IL8 Gene ID: 3576 Accession #: P10145 ELISA Kit: RayBio Human IL-8 ELISA
IL-10 Gene Symbol: IL10 Gene ID: 3586 Accession #: P22301 ELISA Kit: RayBio Human IL-10 ELISA	IP-10 (CXCL10) Gene Symbol: CXCL10 INP10 SCYB10 Gene ID: 3627 Accession #: P02778 ELISA Kit: RayBio Human IP-10 ELISA	MCP-1 (CCL2) Gene Symbol: CCL2 MCP1 SCYA2 Gene ID: 6347 Accession #: P13500 ELISA Kit: RayBio Human MCP-1 ELISA	MIP-1 alpha (CCL3) Gene Symbol: CCL3 G0S19-1 MIP1A SCYA3 Gene ID: 6348 Accession #: P10147 ELISA Kit: RayBio Human MIP-1 alpha ELISA	MIP-1 beta (CCL4) Gene Symbol: CCL4 LAG1 MIP1B SCYA4 Gene ID: 388372 6351 Accession #: P13236 ELISA Kit: RayBio Human MIP-1 beta ELISA
RANTES (CCL5) Gene Symbol: CCL5 D17S136E SCYA5 Gene ID: 6352 Accession #: P13501 ELISA Kit: RayBio Human RANTES ELISA	TGF beta 1 Gene Symbol: TGFB1 TGFB Gene ID: 7040 Accession #: P01137 ELISA Kit: RayBio Human TGF-beta 1 ELISA	VEGF-A Gene Symbol: VEGFA VEGF Gene ID: 7422 Accession #: P15692 ELISA Kit: RayBio Human VEGF-A ELISA

Application Notes

Suggested Application

Kit Components

RayPlex Multiplex Bead Cocktail
1X Assay Diluent
20X Wash Buffer
RayBio^® Serum/Plasma Diluent
Lyophilized Protein Standard Mix
1X Biotinylated Detection Antibody Cocktail
10X PE Conjugate
Flow Cytometer Setup Bead Cocktail
V-bottom 96-well Microplate
Filter 96-well Microplate

Other Materials Required

Orbital 96-well plate shaker (with ability to reach 1000 rpm)
Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
Aluminum foil or 96-well Plate Aluminum Sealers
Distilled water
1.5 ml polypropylene microcentrifuge tubes or similar
Microcentrifuge
If using the Filter 96-Well Microplate (Item 11): Vacuum manifold compatible with a 96-well microplate
Optional: 96-well round bottom plate (to prepare samples)
Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex bead arrays. Get a quote!

Protocol Outline

Prepare Reagents, Standards, and Samples
Add RayPlex Multiplex Bead Cocktail to each well
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluorophore
Analyze with a compatible flow cytometer
Perform data analysis

Featured Products

RayPlex^® Human Mesenchymal Stem Cell Bead Array (13-Plex)

Product Description

Specifications

Product Features

Target Names

Application Notes

Storage/Stability

Have this product in hand?

Featured Products

RayPlex^® Human Mesenchymal Stem Cell Bead Array (13-Plex)

Product Description

Specifications

Product Features

Target Names

Application Notes

Storage/Stability

Have this product in hand?

RayPlex^® Human Mesenchymal Stem Cell Bead Array (13-Plex)

RayPlex^® Human Mesenchymal Stem Cell Bead Array (13-Plex)

Featured Products

RayPlex® Human Mesenchymal Stem Cell Bead Array (13-Plex)

Product Description

Specifications

Product Features

Target Names

Application Notes

Storage/Stability

Documents

Have this product in hand?

Reviews

Ask A Question

Featured Products

RayPlex® Human Mesenchymal Stem Cell Bead Array (13-Plex)

Product Description

Specifications

Product Features

Target Names

Application Notes

Storage/Stability

Documents

Have this product in hand?

Reviews

Ask A Question

RayPlex® Human Mesenchymal Stem Cell Bead Array (13-Plex)

RayPlex® Human Mesenchymal Stem Cell Bead Array (13-Plex)

RayPlex^® Human Mesenchymal Stem Cell Bead Array (13-Plex)

RayPlex^® Human Mesenchymal Stem Cell Bead Array (13-Plex)

RayPlex^® Human Mesenchymal Stem Cell Bead Array (13-Plex)

RayPlex^® Human Mesenchymal Stem Cell Bead Array (13-Plex)