RayBio® C-Series Human Cytokine Antibody Array 1 Kit. Detects 23 Humans Cytokines. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
|---|---|
| Species | Human |
| Number of Targets Detected | 23 |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | Membrane |
| Method Of Detection | Chemiluminescence |
| Design Principle | Sandwich-based |
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| Scroll over each target protein for more information | ||||
| GCSF
| GM-CSF
| GRO alpha/beta/gamma
| GRO alpha (CXCL1)
| IL-1 alpha (IL-1 F1)
|
| IL-2
| IL-3
| IL-5
| IL-6
| IL-7
|
| IL-8 (CXCL8)
| IL-10
| IL-13
| IL-15
| IFN-gamma
|
| MCP-1 (CCL2)
| MCP-2 (CCL8)
| MCP-3 (MARC/CCL7)
| MIG (CXCL9)
| RANTES (CCL5)
|
| TGF beta 1
| TNF alpha
| TNF beta (TNFSF1B)
| ||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Cytokine Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Desai, Mauli B., et al. "Pollen?induced antigen presentation by mesenchymal stem cells and T cells from allergic rhinitis." Clinical & translational immunology 2.10 (2013).
Species:
Human
Sample Type:
Conditioned Media (PBMCs, with pulsed and unpulsed MSCs)
Sze S, Kleijn D, Lai R, Tan E, Zhao H,et al.Elucidating the Secretion Proteome of Human Embryonic Stem Cell-derived Mesenchymal Stem Cells.Mol Cell Proteomics. 2007; 6: 1680-1693
Species:
Human
Sample Type:
Conditioned Media (conditioned or non-conditioned medium)
Im M., Kim SY., Sohn KC., et al. Epigallocatechin-3-gallate suppresses IGF-I-induced lipogenesis and cytokine expression in SZ95 sebocytes. J Invest Dermatol. 2012 Dec;132(12):2700-8. doi: 10.1038/jid.2012.202.
Species:
Human
Sample Type:
Conditioned Media (SZ95 sebocytes)
Norata GD, Tibolla G, Seccomandi PM, Poletti A, Catapano AL. Dihydrotestosterone Decreases Tumor Necrosis Factor-alpha and Lipoploysaccharide-Induced Inflammatory Response in Human Endothelial Cells. J Clin Endocrinol Metab. 2006;91:546-554.
Species:
Human
Sample Type:
Conditioned Media (Human umbilical vein endothelial cell (HUVEC) conditioned media)
Zhang R., Jack GS., Rao N., et al. Nuclear fusion-independent smooth muscle differentiation of human adipose-derived stem cells induced by a smooth muscle environment. Stem Cells. 2012 Mar;30(3):481-90. doi: 10.1002/stem.1023.
Species:
Human
Sample Type:
Conditioned Media (Smooth Muscle cells)
Joner M, Farb A, Cheng Q, Finn A , Acampado E,et al.Pioglitazone Inhibits In-Stent Restenosis in Atherosclerotic Rabbits by Targeting Transforming Growth Factor- and MCP-1.Arterioscler Thromb Vasc Biol. 2007;27:182
Species:
Human
Sample Type:
Conditioned Media (Cell-conditioned media)
Nazzal D., et al. Human thymus medullary epithelial cells promote regulatory T-cell generation by stimulating interleukin-2 production via ICOS ligand. Cell Death and Disease (2014) 5, e1420; doi:10.1038/cddis.2014.377
Species:
Human
Sample Type:
Conditioned Media (mTEC cells)
LaValle CR, Zhang L, Xu S, Eiseman JL, Wang QJ. Inducible silencing of protein kinase D3 inhibits secretion of tumor-promoting factors in prostate cancer. Molecular Cancer Therapeutics 2012;11(7):1389-1399. doi:10.1158/1535-7163.MCT-11-0887.
Species:
Human
Sample Type:
Conditioned Media (PC3, prostate cancer cell lines)
St Hill, Catherine A., Katherine Krieser, and Mariya Farooqui. "Neutrophil interactions with sialyl Lewis X on human nonsmall cell lung carcinoma cells regulate invasive behavior." Cancer 117.19 (2011): 4493-4505.
Species:
Human
Sample Type:
Conditioned Media
Yen Y-T, Liao F, Hsiao C-H, Kao C-L, Chen Y-C, Wu-Hsieh BA. Modeling the Early Events of Severe Acute Respiratory Syndrome Coronavirus Infection In Vitro. J Virol. 2006;80(6):2684-2693.
Species:
Human
Sample Type:
Conditioned Media (THP-1 human monocytic Cell culture supernatant)
- Great productIt works well. Easy to follow instructions.
from Augusta University,
on
- Great productStraight forward protocol with robust and reproducible results.
from Dana Farber Cancer Institute,
on
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