The RayBiotech Urinary Tract Infection (UTI) Multiplex PCR Bacteria Profiling Kit is a ready to use real-time PCR assay for the detection of UTI bacteria DNA in a liquid sample. The kit is based on TaqmanTM DNA Polymerase amplification and fluorochrome-labeled specific-probe reporting. This kit detects 21 common bacteria genomic DNA along with an internal control, RNase P across 7 multiplex real-time PCR panels with 4 different fluorescent probes in each panel. This kit is used for the qualitative in vitro detection of Urinary Tract Infection (UTI) Bacteria nucleic acid from purified DNA samples.

1. Purified human urine DNA: DNA should be purified according to manufacturer protocols
2. DNA preservation solution
3. Fluorescence PCR instrument capable of reading FAM channel (494 nm maximum absorption, 518 nm maximum emission), VIC channel (520 nm maximum absorption, 558 nm maximum excitation), ROX channel (580 nm maximum absorption, 623 nm maximum excitation) and Cy5 (640 nm maximum absorption, 682 nm maximum excitation).
4. Vortex Mixer
5. Microcentrifuge
6. Pipettes
7. Sterile nuclease-free pipette tips (barrier tips recommended) and microfuge tubes
8. Compatible PCR Plate
9. Contact our technical support team for questions about compatibility: techsupport@raybiotech.com

The kit can be stored at -20°C for a period of 12 months prior to opening. After opening, the reagents are valid for at least 6 months if stored at -20°C.